Electrostatic properties of cowpea chlorotic mottle virus ccmv and cucumber mosaic virus cmv were investigated using numerical solutions to the poissonboltzmann equation. The causal agent of common smut is the fungus ustilago maydis. Dissecting the mode of maize chlorotic mottle virus. Lyophilized controls should be reconstituted with the sample extract buffer designated in your test user guide. The disease caused severe economic losses in the midwest. Negative control for maize chlorotic mottle virus mcmv negative control for maize chlorotic mottle virus mcmv click to view a larger image. Maize chlorotic mottle virus mcmv is the only member of the machlomovirus genus in the family tombusviridae. The disease is the result of a combination of two different viruses occurring together in the same plant. The concentration of maize chlorotic mottle virus mcmv was up to 5. We do not use these to store personal information about you. On the basis of significant amino acid sequence homology in the viral polymerase, the machlomovirus genus has been placed within the family tombusviridae. Genomewide association and genomic prediction of resistance. Experimentally, it has been shown that ccmv particles swell in the absence of divalent cations when the ph is raised from 5 to 7. Maize chlorotic mottle virus is easily differentiated from other viruses commonly infecting maize in the western hemisphere maize chlorotic dwarf virus, maize mosaic virus, maize rayado fino virus, maize stripe virusand maize white line mosaic virus, because none of these is mechanically transmissible.
Detection and identification of maize chlorotic mottle virus. Maize chlorotic mottle virus is a plant pathogenic virus. Falk department of plant pathology university of california, davis, ca 95616. Pdf detection and characterization of maize chlorotic. This species also adults of both species overwinter and appear in the field transmits cocksfoot mottle virus cmv 17 and phleum early in the corngrowing season. Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. Continuing to use this website means you agree to our use of cookies. Notes on taxonomy and nomenclature top of page in 1963, a new maize disease was recognized in iowa, usa janson and ellett, 1963, and soon after was shown to be caused by a new virus named maize dwarf mosaic virus mdmv janson et al.
New research on maize lethal necrosis disease in kenya. New model to control deadly maize disease african farming. Symptoms are fine chlorotic yellow streaks developing in the youngest leaves about 10 days after inoculation. Jun 05, 2006 electrostatic properties of cowpea chlorotic mottle virus ccmv and cucumber mosaic virus cmv were investigated using numerical solutions to the poissonboltzmann equation. Similar to its brother virus, cowpea mosaic virus cpmv, ccmv is produced in high yield in plants. The leaves of infected plants develop yellow spots, thus the name chlorotic. The double infection of the two viruses gives rise to what. Maize chlorotic mottle primerdesign ltdvirus tm 150 tests genesig standard kit for general laboratory and research use only 1 quantification of maize chlorotic mottle virus genomes genesig standard kit handbook hb10. Top of page maize chlorotic mottle virus mcmv is the type and only species of the machlomovirus genus lommel, 1995. Our objective was to gain insights into the genetic architecture underlying the resistance to mlnd by genomewide association study gwas and genomic selection. Pdf visual detection of maize chlorotic mottle virus using. Therefore, visual detection of maize chlorotic mottle virus is a potentially useful tool for middle or smallscale corporations and entryexit inspection and quarantine bureaux to detect maize. We report a biosensor based on surface plasmon resonance spr for the selective detection of maize chlorotic mottle virus mcmv. One virus is maize chlorotic mottle virus mcmv, which when combined with another virus sugarcane mosaic virus scmv results in the development of mln disease, leading to.
On the basis of significant amino acid sequence homology in the viral polymerase, the machlomovirus genus has. Detection and characterization of maize chlorotic mottle virus and sugarcane mosaic virus associated with maize lethal necrosis disease in ethiopia. Fao in emergencies app food and agriculture organization. Coat protein gene maize chlorotic mottle virus techne qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic mottle. Machlomovirus is a genus of plant viruses, in the family tombusviridae. New research on maize lethal necrosis disease in kenya the. Machlomovirus is a plant virus with icosahedral virions of 30 nm in diameter containing a singlestranded positivesense rna of. Detection and identification of maize chlorotic mottle. The complete sequence of soybean chlorotic mottle virus dna and the identification of a novel promoter. The crystals exhibit needlelike morphology and diffract to 8a resolution.
Concentration of maize chlorotic mottle virus increased in mixed infections with maize dwarf mosaic virus, strain b. Maize chlorotic mottle virus in hawaiiangrown maize. Detection and characterization of maize chlorotic mottle. All except the last, which is soilborne, are transmitted by either leafhoppers or planthoppers. Pdf visual detection of maize chlorotic mottle virus. The elisa results showed that the two seedlings reacted positively with antibody against maize chlorotic mottle virus mcmv. The first stages of mottling are similar to maize streak virus, but thereafter the two diseases are quite distinct. Our objective was to determine whether assessment of the severity of three symptoms on the leaves. Insight into the threedimensional structure of maize. As a mixed infection with wheat streak mosaic virus wsmv, sugarcane mosaic virus, or maize dwarf mosaic virus, it induces a synergism referred to as corn lethal necrosis. Maize chlorotic mottle virus mcmv is the type species of the genus machlomovirus. General information about maize mottle chlorotic stunt virus mmcsv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience.
The virus is vectored by the larva and adult stages of. Haplothrips nigricornis haplothrips robustus phenacoccus solenopsis helix aspersa grapevine vein necrosis. Cowpea chlorotic mottle virus ccmv, family bromoviridae, rna virus, coloured transmission electron micrograph tem. Apr 20, 2017 detection and characterization of maize chlorotic mottle virus and sugarcanemosaic virus associated with maize lethal necrosis disease in ethiopia. How the pathogen survives and the primary source of inoculum is unknown. This negative control is intended for use in elisa test formats. Cowpea chlorotic mottle virus, tem stock image c032. Brome mosaic virus bmv cereal chlorotic mottle cereal chlorotic mottle virus ccmv corn lethal necrosis maize lethal necrosis disease virus complex maize chlorotic mottle virus mcmv and maize dwarf mosaic virus mdmv a or b or wheat streak mosaic virus wsmv cucumber mosaic. First report of maize chlorotic mottle virus infecting maize zea mays l. Sep 05, 2012 the disease is the result of a combination of two different viruses occurring together in the same plant. Crystalline cowpea chlorotic mottle virus ivan rayment, patrick argos, and john e.
This by itself will cause only minor damage on maize, but when another potyvirus of cereals, such as scmv, wheat streak. Maize chlorotic mottle virus mcmv sugarcane mosaic virus scmv. Help pages, faqs, uniprotkb manual, documents, news archive and biocuration projects. Agdia negative control for maize chlorotic mottle virus mcmv. Cereal chlorotic mottle virus ccmov0overview eppo global. The disease caused severe economic losses in the midwest and southern united states during the 1960s to 1970s. Here, we obtained the cryoem structure of mcmv by single particle analysis with most local resolution at approximately 4 a.
Agdia positive control for maize chlorotic mottle virus. As a mixed infection with wheat streak mosaic virus wsmv, sugarcane mosaic virus, or maize dwarf mosaic virus, it induces a synergism referred to. Johnson department of biological sciences, purdue uniuersity, west lafayette, indiana 47907 recetved may 2, 1977 a new crystal form of cowpea chlorotic mottle virus has been characterized. Common smut occurs in almost every field of corn in nebraska and is not normally a cause for concern except when at high incidence when it can pose a threat to yield or compromises or stalk integrity. Agdia negative control for maize chlorotic mottle virus. Maize chlorotic dwarf virus mcdv, which has a polyhedral particle about 30 nm in diameter, was designated as the type member of the maize chlorotic dwarf virus group in 1981 by the international committee on taxonomy of viruses matthews, 1982. Transmission of maize chlorotic mottle virus by chrysomelid. The leaves of infected plants develop yellow spots, hence the name chlorotic. Though technically a grain, maize kernels are used in cooking as a vegetable or starch.
Maize chlorotic mottle virus how is maize chlorotic mottle. General information about cereal chlorotic mottle virus ccmov0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Quantification of maize chlorotic mottle virus genomes 9 advanced kit handbook hb10. The maize lethal necrosis disease mlnd is a result of a combination of two viruses, the maize chlorotic mottle virus mcmov and any of the cereal viruses in the potyviridae group, like the sugarcane mosaic virus scmv, wheat streak mosaic virus wsmv or maize dwarf mosaic virus mdmv. Indian peanut clump virus maize chlorotic mottle virus pea earlybrowning virus tomato brown rugose fruit virus tomato leaf curl new delhi virus zucchini green mottle mosaic virus 2 five quarantine pests will be deleted from the list. Notes on taxonomy and nomenclature top of page maize chlorotic mottle virus mcmv is the type and only species of the machlomovirus genus lommel, 1995. In the natural host, viral particles can be produced at 12 mg per gram of. The positive samples were further identified by rtpcr method and the 711 bp size target bands were amplified specifically. The results of incidence ratings to assess the host response of maize zea mays l. Tsai fort lauderdale research and education center university of florida, ifas. Soybean chlorotic mottle virus resembles soybean mosaic potyvirus in symptoms and host range, but differs from it in inducing systemic infection in phaseolus vulgariscv. Mlnd is caused by a combination of two viruses, one of which is always maize chlorotic mottle virus mcmv. Maize chlorotic dwarf and related viruses springerlink.
Coat protein gene maize chlorotic mottle virus pcr max ltd tm qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic. Maize lethal necrosis disease mlnd is a new viral disease for africa. Soybean chlorotic mottle virus is easily distingushed from peanut chlorotic leaf streak caulimovirus d. Maize chlorotic dwarf of maize is caused by maize chlorotic dwarf virus mcdv. Detection and characterization of maize chlorotic mottle virus and sugarcanemosaic virus associated with maize lethal necrosis disease in ethiopia. One virus is maize chlorotic mottle virus mcmv, which when combined with another virus sugarcane mosaic virus scmv results in the development of mln disease, leading to serious plant damage. The streaks then coalesce to form chlorotic mottling, which is then followed by leaf necrosis, epinasty. Maize chlorotic mottle virus how is maize chlorotic. The double infection of the two viruses gives rise to. Information is given on the geographical distribution in north america, mexico, usa, hawaii, kansas, nebraska, texas, south america, argentina, brazil, peru. What causes maize lethal necrosis mln arises when maize plants are infected at the same time by two viruses. The maize lethal necrosis disease mlnd caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011.
Our objective was to gain insights into the genetic architecture underlying the resistance to mlnd by genomewide association. Cowpea chlorotic mottle virus, tem stock image c0320373. Cowpea chlorotic mottle virus, known by the abbreviation ccmv, is a virus that specifically infects the cowpea plant, or blackeyed pea. Agdia positive control for maize chlorotic mottle virus mcmv. General information about maize mottlechlorotic stunt virus mmcsv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. The viri ons of this singlestranded rna virus are isometric, and the singlecomponent particles have a smooth spherical or hexagonal shape scheets 2010. Rapid and sensitive detection of maize chlorotic mottle. Pdf first report of maize chlorotic mottle virus infecting.
1192 1074 781 1415 566 348 883 907 1439 1023 1316 457 1104 1495 102 1288 1300 74 650 172 164 1277 162 1466 915 486 1165 25 1501 888 1043 626 265 676 869 1378 312 849 616 372